3D BioSol™ Consulting Services
The best assistance to transform your 3D experience
We provide consulting services for developing and improving protocols using in vitro 3D models. Bonds Biosystems consultants can help you develop and adapt your standard models to 3D technologies. Our goal is to make you successful by designing the most effective experimental approach. We will assist you with on-site experiments (at your home) using BioSol™ products and provide all the support you need to understand our in-house platform and process.
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It's time to mainstream the use of mini organs!
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3D BioSol™ Morphological profiling & High-throughput imaging
Cell painting and organoid quantitative monitoring
Bonds Biosystems provide state-of-the-art organoid painting assays to monitor any effect of your treatment on the Adipose Tissue organoids, as well as a list of quantifications using our proprietary protocols and algorithms to automatically measure several characteristics from the size of the organoid to the lipid droplets dimensions and extracellular matrix density.
For more information, Contact us!
01 - Adipose tissue organoid painting
Lipid droplets, ECM, structural proteins, etc.
02 - Clearing protocols
to allow more in-depth assessments of the core of the organoid
03 - Organoid dimensions
(diameter, area, volume, complexity, etc.) endpoint or overtime
04 - Adipocyte dimensions
(diameter, area, volume, complexity, etc.) endpoint or overtime
05 - Lipid droplets dimensions
(diameter, area, volume, complexity, etc.) endpoint or overtime
06 - Immune fluorescence reactions
standard antibodies, 2D-to-3D adaptations, and new antibodies’ standardization for 3D models
07 - High-throughput confocal imaging and Analysis
sample preparation, imaging and interpretation
3D BioSol™ - Biochemical profiling
In partnership with SBH Sciences, Bonds Biosystems provides many meaningful readouts to make you a case of success.
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01 - Cytokine-induced proliferation
measured by CellTiter 96 Aqueous One Solution Reagent; CellTiter Glo; FACS Analysis (cell cycle analysis)
02 - Cytotoxicity assays
measured by LDH-Glo Cytotoxicity Assay; CytoTox 96 Non-Radioactive Cytotoxicity Assay; CytoTox Glo; Caspase GLO 3/7
03 - Immunoassays
including T-Cell activation; Killing / stimulation assay of PBMC and the utilization of the Lumit Immunoassays method
04 - Neutralizing Antibodies / Binding Antibodies
prepare somehting here
05 - Anti-Drug Antibodies (ADA) assays
06 - Complement Dependent Cytotoxicity (CDCC)
07 - Antibody Dependent Cytotoxicity (ADCC)
e.g., screening therapeutics antibodies
08 - Agonist and Antagonist Screening
e.g., Insulin Receptor Agonist
09 - Receptor inhibitor
e.g., EGF Receptor/ErbB1 inhibitor
10 - In-Vitro Cytokine Release assay / Cytokine Profiling
by ELISA, LUMINEX, ELLA
3D BioSol™ Metabolism & Bioenergetics
Metabolic Functional assays
Bonds Biosystems currently offers a list of adipose tissue-related functional assays to characterize the effects of your target treatments or disease models.
For more information, Contact us!
01 - Adipocyte lipids turnover
a. Lipolysis [Glycerol-Glo™ Assay]
i. β-adrenergic agonists or cAMP analogs that stimulate lipolysis
ii. insulin concentration that inhibits lipolysis (8-bromo-cAMP)
b. Lipogenesis [Triglyceride-Glo™ Assay]
02 - Glucose Uptake
measuring glucose uptake in cells based on the detection of 2-deoxyglucose-6-phosphate (2DG6P) [Glucose Uptake-Glo™ Assay]
03 - Mitochondrial Metabolism
a. oxygen consumption rate (OCR)
b. extracellular acidification rate (ECAR) c. measure long chain fatty acid oxidation (FAO)
(Seahorse Platform)
3D BioSol™ Biomarkers, gene/protein expression & secretions
Molecular assays
In partnership with SBH Sciences, Bonds Biosystems currently offer assays to measure gene and protein expressions as well as several adipose tissue-specific secreted protein assessments.
For more information, Contact us!
01 - RT-qPCR (any gene of interest)
a. Applied Biosystems StepOne PlusTM Real-Time PCR system. We have extensive experience designing and performing qPCR assays using TaqMan and SYBR green detection chemistries
b. Our RT-qPCR workflow involves RNA extraction and QC, cDNA conversion, primer design, and validation (if necessary)
02 - Automated Western Blot
Services provided using PeggySue, WES, and JESS (ProteinSimple)
03 - Biomarkers assessments
Panel for Adiponectin, Leptin, anti- and pro-inflammatory cytokines (other targets also can be contracted, contact us for more information)
ELISA, Flow Cytometry, HTRF®/TR-FRET, Luminex®200, Multiplex Services