3D BioSol™ Adipose Tissue (AT)

Recapitulating the in vivo microenvironment

White adipose tissue (WAT) plays numerous roles in human health and disease. In the absence of adequate WAT models for obtaining a stable culture of fat cells with physiologically relevant phenotypes ex vivo, further insights into adipose biology and adipocyte differentiation are hindered, primarily because no model system fully resembles in vivo adipogenesis.

3D BioSol™ mini-organs are based on the latest 3D culture technology and developed for studying adipocyte biology. 3D BioSol™ Platform is optimized for single scaffold-free spheroid/well culture of human cell models. Our intellectual property can be applied to multiple multi-omics approaches.

The 3D BioSolTM Process allows us to create microorganisms from a variety of visceral and subcutaneous tissue deposits of vascular stromal cells. A further advantage of our 3D BioSolTM Process is that it is plastic- and technology-independent. Consequently, it is independent of any cell plate brand or 3D system.

Optimized isolation of adipose tissue cells

Over the past five years, we have improved the process of isolating representative cells from adipose tissue. Therefore, better cell extraction results can be obtained from the mature adipocyte population and the pelleted stromal vascular fraction (SVF). SVF consists of a heterogeneous population of cells, including circulating blood cells, fibroblasts, pericytes, endothelial cells, and pre-adipocytes. The final step in the SVF isolation procedure is to select the plastic adherent population enriched for "pre-adipocytes."

In addition, we have developed a process for expanding SVF cells, preserving their characteristics and with a high differentiation yield. The result is that more relevant cells are available for storage, and the same batch of cells can be used for more experiments (batch consistency).

3D BioSol™ scaffold-free spheroids and organoids

In the 3D BioSol™  AT Platform, cells are cultured under ultra-low attachment conditions, also known as spheroid culture plates. Virtually no cells attach to the cell culture vessel bottom. 3D spheroids and organoids can be formed without cell-plastic contact through cell-to-cell contact and aggregation. As a result, scaffold-free models are naturally stimulated to produce and secrete extracellular matrix (ECM) without the need for exogenous ECM supplementation.

Cohesive assay formats to streamline integration of 3D BioSol™ AT Mini-organs with NO-Float™  technology

Unlike conventional assay plates, our proprietary NO-Float™ technology provides non-floating and non-adhesive coating conditions simplifying mini-organs handling and multi-step assays while enabling seamless integration of our advanced 3D models into your workflow for cell-based assays.

• Ensure superior compound dosing and prevent unintended mini-organ aspirations
• Ensure long-term preservation of mini-organ morphology and function
• Improve high-content imaging quality and reproducibility
• Speed up mini-organ identification, tracking, and analysis, allowing faster automation

3D BioSol™ AT Mini-organs can be delivered to laboratories around the US in a ready-to-test format with NO-Float™ technology implementation. Our mini-organs are engineered to ensure the transport, handling, and assessment of 3D models safely and efficiently and can be supplied in 96- and 384-well plate formats.

For more information about NO-Float™ technology, Contact us!

3D BioSol™ AT Chemically-induced Obesity (CIO) model combines 3D BioSolTM AT from healthy human donor cells (primary SVF), combined with our proprietary methods, media kit, and cocktails to cause progressive AT dysfunction, including insulin resistance, adipocyte hypertrophy, and fibrosis.

This powerful model includes all of the critical AT cells and inducers needed to replicate the progression of human AT disease in vitro. It is ideal for investigating mechanisms of adipocyte hypertrophy caused by Nutritional Perturbation (AT dysfunction), screening for anti-Obesity drugs, and assessing drug candidates' safety in a relevant disease context.

3D BioSol™ AT Patient-derived Obesity (PDO) consists of 96- or 384-well plates containing 3D BioSol™ AT mini-organs derived from human patients with BMIs ranging from 30 to 60 (and healthy controls with BMIs of 25-27). There is no other platform like 3D BioSol™ AT PDO on the market that offers such versatility.

To obtain plates tailored to your needs, you can customize the number of patients and the BMI range and build mixes with different patients' cells. Since we have a carefully documented case history of a patient-donor that includes information like gender, race, and prevalent disease, this model is ideal for evaluating the effects of compounds considering relevant population characteristics.